normal gastric epithelial cells  (ATCC)

Journal: Hereditas

Article Title: Integrative analysis of Helicobacter pylori-driven stomach adenocarcinoma reveals epigenetic deregulation, immune evasion, and therapeutic resistance

doi: 10.1186/s41065-025-00616-z

Figure Lengend Snippet: Identification and validation of hub genes in H. pylori-associated stomach adenocarcinoma (STAD). A Differentially expressed genes (DEGs) identified from GSE13911 and GSE54129 datasets. B Venn diagram showing 234 overlapping DEGs shared between the two datasets. C Protein–protein interaction (PPI) network constructed from common DEGs using the STRING database. D Top hub genes ranked by degree centrality using the CytoHubba plugin in Cytoscape. E RT-qPCR validation of hub gene expression in eight gastric cancer cell lines versus five normal gastric epithelial cell lines. F Receiver operating characteristic (ROC) curve analysis showing diagnostic performance (AUC values) of THBS2, CTNNB1, COL4A1, and E2F3 in distinguishing cancerous from normal tissues. P-value < 0.05

Article Snippet: A total of eight human STAD cell lines, including MKN45, AGS, SNU-1, SNU-5, NCI-N87, HGC-27, KATO III, and MKN28 and five normal human gastric epithelial cell lines, including GES-1, HFE-145, GES-1 C, GES-1 A, and Hs738.St/Int were purchased from commercial cell repositories including ATCC (American Type Culture Collection, USA) and Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Biomarker Discovery, Construct, Quantitative RT-PCR, Gene Expression, Diagnostic Assay

Journal: Hereditas

Article Title: Integrative analysis of Helicobacter pylori-driven stomach adenocarcinoma reveals epigenetic deregulation, immune evasion, and therapeutic resistance

doi: 10.1186/s41065-025-00616-z

Figure Lengend Snippet: Epigenetic regulation and pathway activity of hub genes in STAD. A Promoter methylation levels of THBS2, CTNNB1, COL4A1, and E2F3 in tumor vs. normal tissues using UALCAN database. B Correlation analysis between promoter methylation and gene expression using GSCA database. C Pathway activity profiles showing functional enrichment: THBS2, COL4A1, and E2F3 activate epithelial–mesenchymal transition (EMT) pathways; CTNNB1 is associated with suppression of hormone receptor pathways; E2F3 additionally activates cell cycle-related signalling. P-value < 0.05

Article Snippet: A total of eight human STAD cell lines, including MKN45, AGS, SNU-1, SNU-5, NCI-N87, HGC-27, KATO III, and MKN28 and five normal human gastric epithelial cell lines, including GES-1, HFE-145, GES-1 C, GES-1 A, and Hs738.St/Int were purchased from commercial cell repositories including ATCC (American Type Culture Collection, USA) and Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Activity Assay, Methylation, Gene Expression, Functional Assay

Journal: Hereditas

Article Title: Integrative analysis of Helicobacter pylori-driven stomach adenocarcinoma reveals epigenetic deregulation, immune evasion, and therapeutic resistance

doi: 10.1186/s41065-025-00616-z

Figure Lengend Snippet: miRNA–mRNA regulatory network and diagnostic value of miRNAs targeting hub genes in STAD. A miRNA–mRNA interaction network constructed using the miRNet database. B Expression analysis of key miRNAs (hsa-miR-15b and hsa-miR-9-2) in STAD vs. normal tissues using UALCAN database. C Kaplan–Meier survival analysis showing no significant association between the expression of hsa-miR-9-3p and hsa-miR-9-5p and overall survival in STAD patients. D RT-qPCR validation of hsa-miR-9-3p and hsa-miR-9-5p expression in eight GC cell lines and five normal gastric epithelial cell lines, confirming their upregulation in cancer. E ROC analysis demonstrating the diagnostic accuracy of hsa-miR-9-3p (AUC = 0.81) and hsa-miR-9-5p (AUC = 0.82) for distinguishing STAD cells from normal counterparts. P-value < 0.05

Article Snippet: A total of eight human STAD cell lines, including MKN45, AGS, SNU-1, SNU-5, NCI-N87, HGC-27, KATO III, and MKN28 and five normal human gastric epithelial cell lines, including GES-1, HFE-145, GES-1 C, GES-1 A, and Hs738.St/Int were purchased from commercial cell repositories including ATCC (American Type Culture Collection, USA) and Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Diagnostic Assay, Construct, Expressing, Quantitative RT-PCR, Biomarker Discovery